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Cell preservation medium

[Packaging Specification]

Specification: 10ml/bottle, 25 servings/box, 400 servings/carton. Record number:粤深械备20230202

  

[Intended use]

This product is suitable for the preservation and transportation of cells derived from the human body, and is solely intended for in vitro analysis and detection purposes, not for therapeutic use.


[Inspection principle]

The cell preservation solution includes pH buffer and osmotic pressure maintainer, which can preserve the integrity of cells without cell lysis or cell swelling.


[Main Components]

The main ingredient of this product is cell preservation solution, which consists of sodium chloride, alcohol, potassium sorbate, EDTA, and other components.


[Storage Conditions and Expiration Date] 

Store at 5~35℃, with an expiration date of 24 months. The reagent is valid for four weeks after opening.


[Applicable instrument]

 Integrated slide preparation and staining machine


[Specimen Requirements]

cervical cells

Collect cervical cell specimens using a disposable cervical brush: Hold the brush handle with your thumb and index finger, insert the tip of the cervical brush into the cervical os, rotate it in one direction for 3-5 revolutions, withdraw the cervical brush, remove the brush head, and place it into a small bottle containing 10ml of fixed preservative solution (if there is excessive mucus or secretions on the patient's cervix, wipe it off with a cotton ball before sampling). Mark the patient's name, age, and outpatient (inpatient) number on the bottle label.

Sputum cell

The patient should first remove saliva, throat secretions, and food residue from their mouth. Then, they should cough up phlegm from the depths of their lungs into the cell preservation bottle provided by our company. Secure the bottle cap tightly and shake it to mix the contents. The bottle label should bear the patient's name, age, and outpatient (inpatient) number. If conditions permit, please use atomized saline inhalation to facilitate expectoration.

hydrothorax and ascites, urine

Let the patient's pleural effusion, ascites, or urine specimen stand at room temperature for half an hour to allow the cells to settle naturally. Suck up 10-50ml of liquid from the bottom of the bottle, centrifuge at 2000 rpm for 4 minutes. If the liquid contains a lot of blood, the centrifuged liquid may separate into three layers. At this point, suck up the second layer of liquid (the boundary between the clear liquid and the blood). If there is very little blood after centrifugation, quickly discard the clear liquid, then stand the centrifuge tube upright. Allow the liquid on the wall of the tube to naturally flow back to the bottom of the tube, mix well, and aspirate into the preservation solution. Mark the patient's name, age, and outpatient (inpatient) number on the bottle label.

 

[Precautions]

1. This product is only for in vitro diagnosis.

2. After collecting cervical cell specimens, the collection brush should be fully immersed in the preservative solution to maximize cell retention.

3. If the cell quantity is found to be insufficient during production, the suction volume of the cell suspension can be appropriately increased.

4. When handling sputum specimens, it is important to dilute and mix them thoroughly.

5. The collected specimens must be kept fresh and fixed promptly to prevent cell autolysis.

6. The preservation solution after specimen collection can be stored at room temperature for 4 weeks without causing cell deformation.


[Warning]

This reagent is intended for in vitro analytical testing and should not be consumed or applied internally or externally to humans or animals. Swallowing may lead to serious consequences; it has certain irritation to eyes and skin. If it accidentally splashes into the eyes, rinse immediately with clean water. Ventilation should be maintained during use.


[References]

1. Ministry of Health, Medical Administration Department of the People's Republic of China. National Clinical Laboratory Practice (M). 3rd Edition. Nanjing: Southeast University Press, 2006

2. Liu Zenghui. Pathological Staining Techniques (M). People's Medical Publishing House


Cell preservation medium
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